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ZipTip® Pipette Tips

For concentrating and purifying femtomoles to picomoles of biomolecules

    ZipTip is a 10 µL pipette tip with a 0.6 or 0.2 µL bed of chromatography media fixed at its end with no dead volume. It is ideal for concentrating and purifying peptides or proteins in seconds prior to mass spectrometry, HPLC, capillary electrophoresis and other analytical techniques.

    The ZipTip pipette tip is simple and easy to use. Place the tip on a single- or multi-channel pipettor, standard 22-gauge blunt-end HPLC needle, or compatible automated liquid handling/sample preparation station. For sample binding, aspirate and dispense through the media several times. Contaminates are then washed away in similar fashion. The concentrated, purified sample is eluted in 1–4 µL of compatible solvent with direct transfer to a mass spectrometer target or vial. For applications requiring smaller elution volumes (e.g., <1 µL), a micro-bed format containing 0.2 µL of media is available.

    The ZipTip pipette tip provides a reproducible, high recovery method for concentrating and purifying femtomoles to picomoles of peptides, proteins and oligonucleotides for improved data quality. For simplified analysis, fractionation of complex peptide mixtures by step elution is also possible.

       • Ready to use
       • Eliminates labor-intensive chromatography techniques

Resins for a Variety of Applications (return to top)

Performance

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Specifications (return to top)

Materials
Pipette TipPolypropylene
C18 and µ-C18 ResinSilica, 15 µm, 200 Å pore size
C4 ResinSilica, 15 µm, 300 Å pore size
SCX ResinStrong cation exchange, 12 µm, 300 Å pore size
Volumes, µL
Pipette tip10
Resin Bed
Capacity
ZipTipC18Typically 5 µg when used with saturating amounts of analyte
ZipTipµ-C18Typically 2.0 µg when used with saturating amounts of analyte
ZipTipC4Typically 3.3 µg when used with saturating amounts of analyte
Dimensions
Tip Length, mm31
Tip ID (top), mm3.375
Tip OD (top), mm5.8
Temperature, °C4–70
pH Range1.5–13.5; 2–12 for 24 hour exposure

Recommended Equipment

    Manual Liquid Handling:
    ZipTip pipette tips are compatible with most 10 µL single-channel pipettors. The Biohit Proline™ electronic multi-channel pipettor is recommended for simultaneously processing eight samples.
    Automated Liquid Handling:
    ZipTip pipette tips are also proven for use on a variety of automated liquid handling systems including:
       • ABI SymBiot® Sample Workstations for Biospectrometry™ with universal cone for ZipTip tips (ABI catalogue no. GEN602987) and rack adapter for ZipTip box
       • Genomic Solutions ProMS™ Sample Preparation Station
       • Bruker Daltonics MAP™ II and II/8 MALDI AutoPrep Systems with rack adapter for ZipTip box (Millipore catalogue no. ST0B DAP TR)
       • Tecan GENESIS Workstation
       • CyBio CyBi™-Well 96 Automated Pipettor


Ordering Information  (return to top)

Select up to 5 products to compare them side by side
Catalogue No.

Compare Catalogue No. Description Qty/Pk  
ZTC18S008 ZipTip with 0.6 µL C18 resin 8 » Add to Cart
ZTC18S096 ZipTip with 0.6 µL C18 resin 96 » Add to Cart
ZTC18S960 ZipTip with 0.6 µL C18 resin 960 » Add to Cart
ZTC04S008 ZipTip with 0.6 µL C4 resin 8 » Add to Cart
ZTC04S096 ZipTip with 0.6 µL C4 resin 96 » Add to Cart
ZTC04S960 ZipTip with 0.6 µL C4 resin 960 » Add to Cart
ZTC18M008 ZipTip with 0.2 µL C18 resin 8 » Add to Cart
ZTC18M096 ZipTip with 0.2 µL C18 resin 96 » Add to Cart
ZTC18M960 ZipTip with 0.2 µL C18 resin 960 » Add to Cart
ZT0MCS008 ZipTip with 0.6 µL metal chelate resin 8 » Add to Cart
ZT0MCS096 ZipTip with 0.6 µL metal chelate resin 96 » Add to Cart
ZTSCXS008 ZipTip with 0.6 µL strong cation resin 8 » Add to Cart
ZTSCXS024 ZipTip with 0.6 µL strong cation resin 24 » Add to Cart
ZTSCXS096 ZipTip with 0.6 µL strong cation resin 96 » Add to Cart


Related Products  (return to top)
ZipTip® Pipette Tips
ZipTip is a 10 µL pipette tip with approximately a 0.6 µL bed of chromatography media fixed at its end such that there is no dead volume. It is ideal for concentrating and purifying peptides, proteins or oligonucleotides in seconds prior to mass spectrometry, HPLC, capillary electrophoresis and other analytical techniques.
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